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differentiation bullekit osteogenic medium  (Lonza)


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    Lonza differentiation bullekit osteogenic medium
    Differentiation Bullekit Osteogenic Medium, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bullekit+osteogenic+medium/differentiation+bullekit+osteogenic+medium/pmc12027874-343-35-40
    Average 90 stars, based on 1 article reviews
    differentiation bullekit osteogenic medium - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: In Vitro Osteoinductivity Assay of Hydroxylapatite Scaffolds, Obtained with Biomorphic Transformation Processes, Assessed Using Human Adipose Stem Cell Cultures
    Article Snippet: OC was obtained using differentiation Bullekit osteogenic medium (Lonza, Milan, Italy), containing osteogenic basal medium (Lonza, Milan, Italy) and osteogenic SigleQuotes, which includes dexamethasone, ascorbate, mesenchymal cell growth supplement, L-glutamine, β-glycerophosphate (Lonza, Milan, Italy) [ , ].



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    Flow cytometric characterization of cell-surface antigen profile of human bone marrow-derived mesenchymal stem cells (hBMSCs) obtained from bone marrow of orthopedic patients. Plots are representative of three distinct analyses, employing hBMSCs derived from a single patient. In each graph, the dark gray histogram plot represents sample stained with the indicated antibody, whereas the light gray histogram plot represents isotype control. CD29, CD73, and CD90 represent hMSC-positive surface markers, while CD14 and CD45 are hMSC-negative surface markers. Phenotypic analysis confirmed that cells tested positive for hMSC markers (CD29, CD73, and CD90) and negative for hematopoietic (CD45) and macrophage (CD14) markers.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Enhanced Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells by a Hybrid Hydroxylapatite/Collagen Scaffold

    doi: 10.3389/fcell.2020.610570

    Figure Lengend Snippet: Flow cytometric characterization of cell-surface antigen profile of human bone marrow-derived mesenchymal stem cells (hBMSCs) obtained from bone marrow of orthopedic patients. Plots are representative of three distinct analyses, employing hBMSCs derived from a single patient. In each graph, the dark gray histogram plot represents sample stained with the indicated antibody, whereas the light gray histogram plot represents isotype control. CD29, CD73, and CD90 represent hMSC-positive surface markers, while CD14 and CD45 are hMSC-negative surface markers. Phenotypic analysis confirmed that cells tested positive for hMSC markers (CD29, CD73, and CD90) and negative for hematopoietic (CD45) and macrophage (CD14) markers.

    Article Snippet: In the OC group, hBMSCs were cultured in hBMSCs differentiation Bullekit TM osteogenic medium (Lonza, Milan, Italy), which contains osteogenic basal medium (Lonza, Milan, Italy) and osteogenic SigleQuotes TM (dexamethasone, ascorbate, mesenchymal cell growth supplement, L -glutamine, and β-glycerophosphate) (Lonza, Milan, Italy) ( ).

    Techniques: Derivative Assay, Staining, Control

    Atomic force microscopy (AFM) analysis of human bone marrow-derived mesenchymal stem cells (hBMSCs) grown on a plastic Petri dish and on Coll/Pro Osteon ® 200 biomaterial. (a) Map of the Young modulus of hBMSCs grown on a plastic Petri vessel. The value of the Young modulus is reported with a Log scale. The substrate is identified by the black region, as also reported on top of the color scale. The red square shows a typical area over which the Young modulus was averaged. (b) Typical example of a force curve obtained on cells on the biomaterial. The horizontal axis reports the tips-sample displacement, and the vertical axis reports the applied force.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Enhanced Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells by a Hybrid Hydroxylapatite/Collagen Scaffold

    doi: 10.3389/fcell.2020.610570

    Figure Lengend Snippet: Atomic force microscopy (AFM) analysis of human bone marrow-derived mesenchymal stem cells (hBMSCs) grown on a plastic Petri dish and on Coll/Pro Osteon ® 200 biomaterial. (a) Map of the Young modulus of hBMSCs grown on a plastic Petri vessel. The value of the Young modulus is reported with a Log scale. The substrate is identified by the black region, as also reported on top of the color scale. The red square shows a typical area over which the Young modulus was averaged. (b) Typical example of a force curve obtained on cells on the biomaterial. The horizontal axis reports the tips-sample displacement, and the vertical axis reports the applied force.

    Article Snippet: In the OC group, hBMSCs were cultured in hBMSCs differentiation Bullekit TM osteogenic medium (Lonza, Milan, Italy), which contains osteogenic basal medium (Lonza, Milan, Italy) and osteogenic SigleQuotes TM (dexamethasone, ascorbate, mesenchymal cell growth supplement, L -glutamine, and β-glycerophosphate) (Lonza, Milan, Italy) ( ).

    Techniques: Microscopy, Derivative Assay

    Stem cell viability, cytoskeleton architecture, and metabolic activity of human bone marrow-derived mesenchymal stem cells (hBMSCs) grown on scaffold. (A) Cytoskeleton architecture of recombinant hBMSC-enhanced green fluorescent protein (eGFP) cultures grown on the biomaterial, at day 14 (T1). (B) In this panel, actin filaments of hBMSC-eGFP grown on the biomaterial at day 21 do not show alteration in the structural organization compared to day 14, suggesting a good compatibility of the scaffold (magnification 20×). (C) hBMSC metabolic activity measured by AlamarBlue assay at days 14 and 21 of cultivation on the biomaterial. Statistically significant differences are evident between hBMSCs grown on biomaterials at days 14 and 21 (* p < 0.05). Tissue culture polystyrene (TCPS) exhibited the highest value in cell viability at day 21.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Enhanced Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells by a Hybrid Hydroxylapatite/Collagen Scaffold

    doi: 10.3389/fcell.2020.610570

    Figure Lengend Snippet: Stem cell viability, cytoskeleton architecture, and metabolic activity of human bone marrow-derived mesenchymal stem cells (hBMSCs) grown on scaffold. (A) Cytoskeleton architecture of recombinant hBMSC-enhanced green fluorescent protein (eGFP) cultures grown on the biomaterial, at day 14 (T1). (B) In this panel, actin filaments of hBMSC-eGFP grown on the biomaterial at day 21 do not show alteration in the structural organization compared to day 14, suggesting a good compatibility of the scaffold (magnification 20×). (C) hBMSC metabolic activity measured by AlamarBlue assay at days 14 and 21 of cultivation on the biomaterial. Statistically significant differences are evident between hBMSCs grown on biomaterials at days 14 and 21 (* p < 0.05). Tissue culture polystyrene (TCPS) exhibited the highest value in cell viability at day 21.

    Article Snippet: In the OC group, hBMSCs were cultured in hBMSCs differentiation Bullekit TM osteogenic medium (Lonza, Milan, Italy), which contains osteogenic basal medium (Lonza, Milan, Italy) and osteogenic SigleQuotes TM (dexamethasone, ascorbate, mesenchymal cell growth supplement, L -glutamine, and β-glycerophosphate) (Lonza, Milan, Italy) ( ).

    Techniques: Activity Assay, Derivative Assay, Recombinant, Alamar Blue Assay

    Osteogenic markers in human bone marrow-derived mesenchymal stem cells (hBMSCs) cultured on the biomaterial. (A) Alizarin red staining at day 21 is shown in the panel, in experimental conditions tested. Magnification 4×. (B) To quantify the amount of alizarin red staining in hBMSCs grown on the biomaterial, cells were eluted with 20% methanol and 10% acetic acid in a water solution and measured spectrophotometrically at 450 nm. Matrix mineralization data are reported as optical density and shown in the graph. (C) Detection of osteopontin and osteocalcin proteins by immunofluorescence staining in hBMSCs, at day 21. Symbols indicate statistical significance (* p < 0.001). Magnification 20×. (D) The temporal pattern of osteocalcin protein levels detected at different time points, i.e., at days 14 and 21, was quantified by ELISA. Osteocalcin protein was reported as ng of osteocalcin/1 μg of total protein.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Enhanced Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells by a Hybrid Hydroxylapatite/Collagen Scaffold

    doi: 10.3389/fcell.2020.610570

    Figure Lengend Snippet: Osteogenic markers in human bone marrow-derived mesenchymal stem cells (hBMSCs) cultured on the biomaterial. (A) Alizarin red staining at day 21 is shown in the panel, in experimental conditions tested. Magnification 4×. (B) To quantify the amount of alizarin red staining in hBMSCs grown on the biomaterial, cells were eluted with 20% methanol and 10% acetic acid in a water solution and measured spectrophotometrically at 450 nm. Matrix mineralization data are reported as optical density and shown in the graph. (C) Detection of osteopontin and osteocalcin proteins by immunofluorescence staining in hBMSCs, at day 21. Symbols indicate statistical significance (* p < 0.001). Magnification 20×. (D) The temporal pattern of osteocalcin protein levels detected at different time points, i.e., at days 14 and 21, was quantified by ELISA. Osteocalcin protein was reported as ng of osteocalcin/1 μg of total protein.

    Article Snippet: In the OC group, hBMSCs were cultured in hBMSCs differentiation Bullekit TM osteogenic medium (Lonza, Milan, Italy), which contains osteogenic basal medium (Lonza, Milan, Italy) and osteogenic SigleQuotes TM (dexamethasone, ascorbate, mesenchymal cell growth supplement, L -glutamine, and β-glycerophosphate) (Lonza, Milan, Italy) ( ).

    Techniques: Derivative Assay, Cell Culture, Staining, Immunofluorescence, Enzyme-linked Immunosorbent Assay

    Genes found to be up- or down-regulated in human bone marrow-derived mesenchymal stem cells (hBMSCs) grown on the scaffold at day 21.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Enhanced Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells by a Hybrid Hydroxylapatite/Collagen Scaffold

    doi: 10.3389/fcell.2020.610570

    Figure Lengend Snippet: Genes found to be up- or down-regulated in human bone marrow-derived mesenchymal stem cells (hBMSCs) grown on the scaffold at day 21.

    Article Snippet: In the OC group, hBMSCs were cultured in hBMSCs differentiation Bullekit TM osteogenic medium (Lonza, Milan, Italy), which contains osteogenic basal medium (Lonza, Milan, Italy) and osteogenic SigleQuotes TM (dexamethasone, ascorbate, mesenchymal cell growth supplement, L -glutamine, and β-glycerophosphate) (Lonza, Milan, Italy) ( ).

    Techniques:

    PCR array analyses of osteogenic genes expression in human bone marrow-derived mesenchymal stem cells (hBMSCs) grown on Coll/Pro Osteon ® 200 compared with tissue culture polystyrene (TCPS) reported at day 21. (A) Graphical representation through heat map of the mRNA expression in hBMSCs. The fold-change values (Log 2 < 1 or > 1) is represented as up-regulated (red) and down-regulated (green) genes in hBMSCs grown on Coll/Pro Osteon ® 200 compared with the TCPS. (B) Graphical representation through bars of the mRNA expression in hBMSCs grown on Coll/Pro Osteon ® 200 compared with TCPS. Over-expressed genes ( n = 16), at day 21, were transcription factor Sp7 (SP7); GLI family zinc finger 1 (GLI); TNF superfamily member 11 (TNFSF11); bone morphogenetic proteins 2 and 3 (BMP2 and BMP2 3); colony-stimulating factor 3 (CSF3); SMAD family member 3 (SMAD3); Collagen type II alpha 1 ( COL2A1 ); matrix metallopeptidases 9 and 10 (MMP9 and MMP10); CD36 molecule (CD36); transforming growth factor, beta 3 (TGFB3)/RANKL; secreted phosphoprotein 1 (SPP1), Noggin (NOG); integrin, alpha M (ITGAM); intercellular adhesion molecule 1 (ICAM1). Down-regulated gene ( n = 16) were cartilage oligomeric matrix protein (COMP); insulin growth factors 1 and 2 (IGF1, IGF2); SMAD family member 1 (SMAD1); runt-related transcription factor 2 (RUNX2); cathepsin K (CTSK), alkaline phosphatase (ALP); collagen type IV alpha 1 (COL5A1); fibroblast growth factor receptor 2 (FGFR2); biglycan (BGN); cadherin 11, type 2 (CDH11); colony-stimulating factor 2 (CSF2); twist family BHLH transcription factor 1 (TWIST1); bone morphogenetic protein receptor type II (BMPR2); Collagen type I alpha 1 (COL1A1); insulin growth factor 1 receptor (IGF1R); insulin growth factor 2 (IGF2).

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Enhanced Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells by a Hybrid Hydroxylapatite/Collagen Scaffold

    doi: 10.3389/fcell.2020.610570

    Figure Lengend Snippet: PCR array analyses of osteogenic genes expression in human bone marrow-derived mesenchymal stem cells (hBMSCs) grown on Coll/Pro Osteon ® 200 compared with tissue culture polystyrene (TCPS) reported at day 21. (A) Graphical representation through heat map of the mRNA expression in hBMSCs. The fold-change values (Log 2 < 1 or > 1) is represented as up-regulated (red) and down-regulated (green) genes in hBMSCs grown on Coll/Pro Osteon ® 200 compared with the TCPS. (B) Graphical representation through bars of the mRNA expression in hBMSCs grown on Coll/Pro Osteon ® 200 compared with TCPS. Over-expressed genes ( n = 16), at day 21, were transcription factor Sp7 (SP7); GLI family zinc finger 1 (GLI); TNF superfamily member 11 (TNFSF11); bone morphogenetic proteins 2 and 3 (BMP2 and BMP2 3); colony-stimulating factor 3 (CSF3); SMAD family member 3 (SMAD3); Collagen type II alpha 1 ( COL2A1 ); matrix metallopeptidases 9 and 10 (MMP9 and MMP10); CD36 molecule (CD36); transforming growth factor, beta 3 (TGFB3)/RANKL; secreted phosphoprotein 1 (SPP1), Noggin (NOG); integrin, alpha M (ITGAM); intercellular adhesion molecule 1 (ICAM1). Down-regulated gene ( n = 16) were cartilage oligomeric matrix protein (COMP); insulin growth factors 1 and 2 (IGF1, IGF2); SMAD family member 1 (SMAD1); runt-related transcription factor 2 (RUNX2); cathepsin K (CTSK), alkaline phosphatase (ALP); collagen type IV alpha 1 (COL5A1); fibroblast growth factor receptor 2 (FGFR2); biglycan (BGN); cadherin 11, type 2 (CDH11); colony-stimulating factor 2 (CSF2); twist family BHLH transcription factor 1 (TWIST1); bone morphogenetic protein receptor type II (BMPR2); Collagen type I alpha 1 (COL1A1); insulin growth factor 1 receptor (IGF1R); insulin growth factor 2 (IGF2).

    Article Snippet: In the OC group, hBMSCs were cultured in hBMSCs differentiation Bullekit TM osteogenic medium (Lonza, Milan, Italy), which contains osteogenic basal medium (Lonza, Milan, Italy) and osteogenic SigleQuotes TM (dexamethasone, ascorbate, mesenchymal cell growth supplement, L -glutamine, and β-glycerophosphate) (Lonza, Milan, Italy) ( ).

    Techniques: Expressing, Derivative Assay